| Arylamine
N-Methyltransferase Activity from
Cholestatic Liver after Common Bile Duct Ligation in Rats |
| 쥐 담즙울체간에서의 Arylamine N-Methyltransferase의 활성도 |
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곽춘식; 주일; 문교철 |
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| Abstract |
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Changes of arylamine N-
methyltransferase (AMT) activity in
cholestatic rat liver were studied. Cytosolic; mitochondrial and microsomal AMT
activities were determined in cholestatic rat livers induced by common bile duct
(CBD) ligation over a period of forty two days. The enzyme activity in serum; and
Km and Vmax values of the hepatic enzyme were also measured. The activities of
mitochondrial and microsomal AMT in cholestatic rat liver were found to be
significantly increased between the first and the seventh day; and the first and
the twenty eighth day; respectively after CBD ligation. However; the cytosolic
AMT activity did not change. The AMT activity in serum was significantly
increased between the first and the twenty eighth day after CBD ligation. The
Vmax values of the mitochondrial and microsomal AMT in cholestatic rat liver
were significantly increased at the seventh day after CBD ligation. On the other
hand; the Km values of the above hepatic enzyme did not vary in all the
experimental groups. Therefore; the above results indicate that the biosynthesis of
AMT was increased in cholestatic rat liver. The elevated activity of serum AMT is
most likely caused by increased hepatocytes membrane permeability due to
cholestasis mediated liver cell necrosis. |
| Key Words:
Arylamine
N-methyltransferase, Cholestatic liver, Common bile
duct ligation |
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