Effect of Common Bile Duct Ligation on The Serum and Liver Alanine Aminotrazisferase Activities in Ethanol Intoxicated Rats

Keimyung Medical Journal 1989;8(1):113-121.

Ethanol중독 흰쥐에서 총담관결찰이 혈청 및 간의 Alanine Aminotransferase 활성에 미치는 영향

김여희; 곽춘식; 정성광

Abstract

This study was intended to observe the effect of common bile duct ligation on serum and liver alanine aminotransferase(ALT) activities in rats suffering from acute and chronic intoxication of ethanol. For chronic intoxication of ethanol; the rats were fed 5%(v/v) ethanol instead of water for 60days. Common bile duct of the same group of rats were ligated with ethanol constantly being fed. The rats were killed on the 1st’ 2nd; 3rd; 7th and 14th days of the procedure to measure the cytosolic and mitochondrial ALT activities of the liver. The serum ALT activities were also measured. For acute intoxication of ethanol 4g of ethanol were administered orally per kg of body weight as a single dose. The rats were killed at the 1.5th and 24th hours of the procedure for study. On the 14th day following common bile duct ligation; the rats were acutely intoxicated with ethanol to; be killed at the 1.5 the and 24th hours for measuring the activities of the above enzymes. In terms of rats liver cytosolic and mitochondrial ALT activities; no significant increase was shown in either chronically intoxicated ethanol group or acutely intoxicate ethanol group. The liver cytosolic and mitochondrial ALT activities of the common bile duct ligation groups showed a significant decrease at the 7th and the 14th days of the ligation. And the groups of acute intoxication with ethanol which was done after 14days