Cytogenetic Analysis on Primary Squamous Cell Carcinoma in Human

Keimyung Medical Journal 1989;8(1):54-60.

인체편평상피세포의 세포유전학적 분석

권진우; 편영식

Abstract

Cytogenetic analysis was performed in human squamous cell carcinoma untreated with drugs or radiation. Tumor was disaggregated with mechanical and enzymatic methods. Long-term trypsinization(4;c? 24 hours) reproduced more cell yields than short-term trypsinization(37'c; 30-60 minutes) however; the frequency of metaphases was no significance. Disaggregated cells were treated with cokemid (5ug/ml) for 3 hours. Recognizable metaphase chromosomes could be obtained from conventional preparation of chromsomes. The results were summariijed as follows: Chromosomal range was 42 to 50; but one cell line showed near-tetraploid. There were no findings of specific chromosomal abnormalities such as structural rearrangement or marker chromsome. Nonrandom chromosomal losses were found at chromosome #10; #12; #15; #17;#22. However; these chromosomal losses are of no significance since these cells will die soon. Specific findings in this study were trisomy #1; #7. It is known that ras oncogens is located in the chromosome #1 and raf-2 oncogene in the chromosome #7. Further sutdy is in need to clarify the relation between these oncogenes and trisomy in squamous cell carcinoma