Transfer of Immunity to Clonorchis sinensis in Golden Hamsters Given Peritoneal Exudate Cells Sensitized by Metabolite of Its Excysted Metacercariae

Keimyung Medical Journal 1988;7(2):193-203.

간흡충 탈양유충의 대사산물로 감작한 햄스터 복강삼출세포의 면역이입

조원현; 최동익; 박영관

Abstract

In order to evaluate the role of peritoneal exudate cells (PEC) and serum in the transfer of immunity against Clonorchis sinensis;the donor golden hamsters were sensitized with the metabolite of excysted metacercaria of C. sinensis* E DH; and those of Group 4 were injected IP with 1.0ml of serum from Group I DH. The hamsters of Group 5 were injected IP with 5X106 PEC and those of Group 6 were administered IP with 1.0ml of serum from non-sensitized controls. Seven days after primary sensitization; RH were challenged orally with 20 metacercariae and Eggs per Gram (EpG) of fecal samples were counted from 13 to 49 days after challenge. The RH were killed 50 days after challenge; and the transfer of immunity to the RH was estimated by significant differences in mean worm burdens and plaque forming cells per spleen between sensitized and non-sensitized groups. The eggs of C. sinensis appeared in the 14 days after challenge in Groups 2;4;5 and 6; and in the 15 days in Groups 1 and 2 by the formalin-ether sedimentation; and EpG noted in the 15 and 16 days by Stoll's egg counting techniques. No significant difference was observed in EpG between the sensitized groups and the nonsensitized controls. The hamsters given sensitized PEC harbored fewer flukes than the control groups. The differences in mean worm burdens between the groups given PEC and the control groups were significant by the paired if一test. Whereas no significant differences were found in mean worm burdens between the groups given immune serum and the control group. There were more plaque forming cells in the groups given immune serum than in those given sensitized PEC. However; no plaque forming cells were encountered in the non-sensitized groups. The donor hamsters (DH) were divided into two groups. One group (Group I) was sensitized with two injections of the admixture of the metabolic products of the excysted C. sinensis metacercariae and Freund's incomplete adjuvant into footpads at two weeks interval; and the other group (Group IT) injected intraperitoneally with the admixture. Two weeks after sensitization; the PEC and serum were collected from the DH under deep anesthesia. Recipient hamsters (RH) were divided into 6 groups. The hamsters of Group 1 were injected intraperitoneally (IP) with 5X105 PEC from Group I DH; those of Group 2 were injected IP with 1.0ml of serum from Group I DH; those of Group 3 were injected IP with 5X105 PEC from Group