5'-Nucleotidase and Gamma-Glutamyl Transpeptidase Activities in the Regenerating Rat Liver

Keimyung Medical Journal 1987;6(2):241-251.

흰쥐 재생간의 5´-Nucleotidase 및 Gamma-Glutamyl Transpeptidase의 활성치

안광욱; 곽춘식

Abstract

A study was made on the changes in the activities of the followings during 6 post-ope;if Eitive. days: Plasma membrane; microsomal; mitochondrial; nuclear and cytosolic 5'-nucleo^idase and gamma-glutamyl transpeptidase of regenerating rat livers; and rat serum 5'-nucleotidase and gamma-glutamyl transpeptidase after 70% (median and left lateral lobes) partial hepatectomy. The activities of alanine aminotransferase in both serum and cytosol of regenerating; rat liver were also measured. ? The activities of 5'-nucleotidase in the normal rats hepatic subcellular fractions were: ;59.20+ 11.35 (n mol Pi/mg protein/min) in the plasma membrane; 52.86+3.88 in the microsome; 10.56+ 2.12 in the mitochondria; .11.48±3.62 in the nuclei; and 3.55±0.54 in the cytosol. Those of gamma-glutamyl transpeptidase were: 1.96±0.46 (n mol p-nitroaniline/mg protejn/min) in the plasma membrane; 3.38±0.62in the microsome; 0.88+0.12 in the mitochondria; 1.40+0.13 in the nuclei; and 0.76+0.12 in the cytosol. Normal activity of alanine aminotransferase was 330+ 56 Karmen unit/mg protien in the rat hepatic cytosol. After partial hepatectomy in the rats; activities of plasma membrane bound gamma-glutamyl transpeptidase in regenerating livers significantly increased in the span between the second and the third days but plasma membrane bound 5'-nucleotidase showed no change. The activity of microsomal 5'-nucleotidase in the regenerating liver tremendously increased.from; the second to the sixth days after partial hepatectomy. And the. activity of hepatic microsomal gamma-glutamyl transpeptidase also elevated from the second and the third dayp fifter partial hepatectomy. The activity of mitochondrial 5'-nucleotidase in the regenerating liver decreased between the third and the sixth days after partial hepatectomy. And the activity of hepatic microsomal gamma-glutamyl trsnspeptidase also elevated from the second and; the third days after partial hepatectomy. The activity of mitochondrial 5'-nucleotidase in the regenerating liver decreased between the third and the sixth days of partial hepatectomy. The hepatic nuclear and cytosolic 5'-nucleotidase decreased in the regenerating stage. The decreased values were seen after two days in cytosolic 5'-nucleotidase and after three days in nuclear 5'-nucleotidase following the operations. However; no significant changes in hepatic mitochondrial; nuclear and cytosolic gamma-glutamyl transpeptidase were noted through out the experiments. The activity of serum alanine aminotransferase markedly elevated from 12 hours to three days after partial hepatectomy; but serum 5'-nucleotidase and gamma-glutamyl transpeptidase activities remained normal.