Scanning electron microscopic observations on the hepatocytes and intrahepatic microcirculation of the rat treated with carbon tetrachloride

Keimyung Medical Journal 1986;5(2):204-211.

사염화탄소 투여로 인한 간세포 상해와 간내 미세혈관계의 변화에 대한 주사전자현미경적 관찰

채종민

Abstract

Intracellular structures of hepatocytcs and the hepatic microcirculation were studied rats intoxicated with carbon tetrachloride by scanning electron microscopy. Liver tissues were treated by the DMSO cracking method and aldehyde-prefix osmium-DMSO-osmium method. A rapid, easy and reproducible method to prepare plastic corrosion casts of the hepatic microcirculation employed Mercox CL-2B. The hepatocytes around the portal tracts showed minimal abnormalities. However, swollen foamy hepatocytes in intermediate zone and central zone had numerous vesicles and vacuoles, which correspond to dilated cistemae of rough endoplasmic reticula (RERj and fat droplets observed by transmission electron microscopy and aldehyde prefix O-D-O method. And also, disorder of intrahepatic microcirculation was obsorved U he irregr/lar-shaped sinusoids and distended space of Disse in acute hepatic injury. This abnormal microcirculation was wt'!] demonstrated three-dimensionally by scanning clcctron microscopy of corrcsioii easts.