| Combination of Ischemic Preconditioning and Postconditioning
in Isolated and Intact Rat Hearts: Does it Work or Not? |
| Combination of Ischemic Preconditioning and Postconditioning in Isolated and Intact Rat Hearts: Does it Work or Not? |
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In Gyu Yeo; Hyoung Seob Park; Sang Woong Choi; Han Byul Kim; Jae Pil Lee; In Cheol Kim; Jeong Eun Lee; Hyuck Jun Yoon; Hyung Seop Kim; Chang Wook Nam; Seung Ho Hur; Yoon Nyun Kim; Kwon Bae Kim; Young Ho Jang |
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| Abstract |
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Ischemic preconditioning (Pre) phenomenon was well known as cardioprotective effects and its
signaling pathways leading to myocardial protection activated by NO/cGMP pathways. In the
meantime; the ischemic postconditioning (Post) in which brief intermittent repetitive interruptions
to reperfusion at the onset of timely reperfusion after a prolonged period of ischemia also
reduced myocardial injury to an extent comparable to Pre. In isolated working heart experiment;
all hearts were subjected to 30 min of regional ischemia and 120 min of reperfusion: Control; no
intervention; Pre; 3 cycles of 5-min of global ischemia and 5 min of reperfusion before the
ischemic insult; Post; 6 cycles of 10 sec of reperfusion followed by 10 sec of global ischemia
immediately after the index ischemia; Both (Pre + Post). Infarct size; coronary flow (CF); heart
rate (HR); left ventricular developed pressure (LVDP); and +dP/dt were measured. The infarct
size in the Pre (n=7; 18.5% ± 2.1%); Post (n=7; 19.1% ± 1.5%); and Both (n=10; 21.7% ±
1.7%) groups was significantly decreased compared with Control (n=7; 29.9% ± 1.7%).
However; there was no additive effect when Pre and Post were combined. There were no
significant differences in baseline CF; HR; LVDP; +dP/dt among the groups; and no significant
differences in HR; LVDP; +dP/dt in all groups after 2 hours of reperfusion. In conclusion; Pre and
Post; Both group improved cardioprotective effects than Control group. However; combination of
ischemic preconditioning and postconditioning showed no additive cardioprotective effects in isolated rat heart models. |
| Key Words:
Ischemic postconditioning,
Ischemic preconditioning, Myocardial ischemia |
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