Cytokine Array in Patients with End-Stage Renal Disease |
말기신부전 환자에서 Cytokine 발현 |
성정훈; 하은영; 김여희; 최혜정; 손정남; 황은아; 한승엽; 박성배; 김현철; 문교철 |
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Abstract |
Patients with end-stage renal disease (ESRD) present an extremely high mortality
rate. The leading hypothesis to explain this high mortality risk is that ESRD patients are exposed
to a number of traditional risk factors for cardiovascular disease including atherosclerosis which is
known as an active inflammatory process. The inflammatory process is mediated by several
mediators including cytokines. Among the several mediators; the cytokines have received
particular attention in recent years. The purpose of this study is to profile the expression of
cytokines related to atherosclerosis and inflammation in patients with ESRD; Cytokine antibody
array was performed using serum from patients treating with hemodialysis and peritoneal dialysis.
And microarray for 4;800 genes to detect gene expression was performed using ROS 17/2.8 cells
incubated with 20 mmol/L cyanate. Intercellular adhesion molecule-1; interleukin 1 soluble
receptor type 1; monocyte chemoattractant protein 1; macrophage-colony stimulating factor;
macrophage inflammatory protein; platelet-derived growth factor; RANTES (Regulated upon
Activation; Normal T-cell Expressed and Secreted); transforming growth factor beta 1; tumor
necrosis factor alpha; eotaxin; interleukin-6 soluble receptor; interleukin 8; interleukin 10;
interleukin 16; and tissue inhibitor of metalloproteinases-2 were increased in their expression in patients with ESRD. According to these results; cardiovascular complications may be related with
the increased expression of the some cytokines. |
Key Words:
Cytokine array, Dialysis, End-stage renal disease |