| Detection of cyanate, nitrate and carbamylated proteins
in the peritoneal dialysate |
| Detection of cyanate, nitrate and carbamylated proteins
in the peritoneal dialysate |
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Hyun Chul Kim;? Sung Bae Park;? Sung Yeup Han; Eun A Hwang;? Hye Jung Choi;? Son Jung Nam; Eunyoung Ha;? You Hee Kim;? Kyo Cheol Mun |
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| Abstract |
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Cyanate is known to contribute to peritoneal injury with morphological changes in
the peritoneum. Against carbamylation by cyanate; some proteins including albumin are known to
play a protective role by self-carbamylation. Although many studies about cyanate and
carbamylated proteins; there are no reports about the detection of the cyanate and carbamylated
protein in peritoneal dilaysate. In this study; the cyanate; nitrate; and the carbamylated protein
levels in peritoneal dialysate were measure to determine the existence of the uremic toxins in
peritoneal dialysate. Cyanate was determined using by the isocratic size exclusion
chromatography. Proteins such as albumin; globulin; and hemoglobin were carbamylated with
sodium cynate. For the determination of the carbamylated protein in peritoneal dialysate; we
performed HPLC system. Nitrite concentration was also assessed in the peritoneal dialysate. In
this study; cyanate and nitrate were detected in the peritoneal dialysate. However; carbamylated
proteins are not detected in the peritoneal dialysate. According to this study; peritoneal damage
during PD may caused by continuous contact with uremic toxins including cyanate and nitrate
without proper protection such as proteins. |
| Key Words:
Carbamylation, Cyanate, Nitrate, Peritoneal dialysis |
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