Effects of Indocyanine Green on Cultured Retinal Pigment Epithelial Cells |
Indocyanine Green이 배양된 망막색소상피세포에 미치는 영향 |
백철민; 김광수; 김유철 |
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Abstract |
This study was designed to investigate effects of indocyanine green (ICG) on
cultured retinal pigment epithelial cells; used in macular hole surgery to improve visualization of
the internal limiting membrane and facilitate complete dissection of the internal limiting membrane.
After cultured porcine retinal pigment epithelial cells were exposed to ICG; the morphological
change; toxicity and effect on cell proliferation was compared and analyzed with the control group
exposed to balanced salt solution (BSS??). Cultured porcine retinal pigment epithelial cells were
seeded and grown in 24-well and 96-well plates. Cells were exposed to BSS??; 0.1%; 0.5% and
1.0% concentration of ICG for 30 seconds; 1 minute; 5 minutes and 10 minutes. Cells were
evaluated by phase-contrast microscopy; MTT assay or transmission electron microscopy.
Retinal pigment epithelial cells exposed to ICG showed no morphological change except slight
irregularity in cell size and demonstrated no toxicity and inhibitory effect on cell proliferation. But;
among those exposed to over 0.5% concentration of ICG for over 5 minutes; retinal pigment
epithelial cells proliferating to occupy the central defect area showed tendency to be transformed
to fibroblast-like cells. Cytoplasmic vacuoles were observed in proliferating cells in ICG treated
groups on transmission electron microscopy. These results suggest that ICG with a concentration
of 1.0-5.0 mg/mL (0.1 - 0.5%) for about one minute exposure time appears to be safe to retinal
pigment epithelial cells but loss of retinal pigment epithelial cells during macular hole surgery
assisted by ICG may influences visual outcomes due to poor differentiation of proliferating cells. |
Key Words:
Indocyanine Green, Macular hole, Retinal pigment epithelial cells |