Immunohistochemistry Using Tissue Arrays in the Medical Research

Keimyung Medical Journal 2005;24(2):112-119.

의학 연구에서 조직배열 방법을 이용한 면역조직화학법

강유나

Abstract

Tissue array is an analytic method of multiple tissues from many patients in one slide. Because of high efectiveness; tissue array has been actively used recently in pathology research. It is possible to collect various paraffin block and to make tissue array slides from human normal and tumor tissue; fetal tissue; autopsy; and experimental animal tissue as well as variable cell lines. Tissue array slide for cell lines is made for cultured cell lines after embedding in agarose like tissue parpffin block and it can be used in immunofistochemical stain. Uses of tissue array slide is variable; mostly protein expression of immunohistochemical stain; mRNA expression of in situ hybridization or nuclear DNA expression of fluorescent in situ hybridization. So; it will be very helpful to use many objects (tissue specimens; or antibodies) at one time in the medical research using experimental animals or clinical biopsy tissue. In the future; potential applications of tissue arrays will be more extensive in a number of fields of molecular biomedical research. In this review; I give a brief overview of tissue array technology; research uses including immunohistochemical stain and microscopic scoring process; and discuss potential applications of tissue arrays in the practice of pathology.

Key Words: Immunohistochemical stain, Paraffin block, Tissue array, Tissue array slide