Induction of Hepatic 
Glyoxalase-I by Taurocholate in Rats

Keimyung Medical Journal 2001;20(1):1-11.

Induction of Hepatic Glyoxalase-I by Taurocholate in Rats

쥐에서 Taurocholate 부하에 의한 간의 Glyoxalase I의 유도

정상호; 김여희; 곽춘식

Abstract

The possible mechanisms of increased glyoxalase I (GLO-I) activity in cholestatic rat liver and serum were studied. These hepatic and serum enzyme activities were determined from the experimental rats with choledocho-caval shunt (CCS) or bile duct obstruction (BDO). The Michaelis-Menten constants in this hepatic enzyme were also measured. The activities of hepatic and serum GLO-I as well as the Vmax value of this hepatic enzyme were found to be increased significantly in both CCS plus taurocholic acid (TCA) injected group; and BDO plus TCA injected group than in each control group such as CCS alone and BDO alone. In addition; these serum and hepatic enzyme activities did not change in both the CCS plus tauroursodeoxycholic acid injected group and the BDO plus tauroursodeoxycholic acid injected group. On the other hand; the value of Km of the above hepatic enzyme did not change in any experimental group. Above results suggest that TCA induce the biosynthesis of GLO-I in the liver. The elevated activity of the serum GLO-I is believed to be caused by the increment of membrane permeability of hepatocytes upon TCA mediated liver cell necrosis.

Key Words: Bile duct obstruction, Choledocho-caval shunt, GlyoxalaseⅠ, Taurocholic acid, Tauroursodeoxycholic acid