| Development of
Convenient Shuttle Vectors for Generation of Recombinant Adenoviruses |
| 재조합 아데노 바이러스 생산을 위한 효과적인 Shuttle 벡터의 개발과 검증 |
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박종구 |
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| Abstract |
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Recombinant adenovirus is an
attractive vehicle to deliver a foreign gene for the virus can infect many cell types including non-dividing cells. Production of a recombinant adenovirus requires co?transfection of a shuttle vector which harbors a gene of interest and a; rescue vector. Adenoviral shuttle vectors currently in use contain limited numbers of cloning sites for insertion of a foreign gene. They also lack a promoter and a signaling sequence for the poly(A) tail . Therefore; we set out to construct two adenoviral shuttle vectors; pAd-YC and pAd-YC2; in an effort to facilitate cloning and expression of foreign genes. pAd-YC contains the CMV promoter and multiple cloning sites but without a poly (A) signal sequence. pAd-YC2 contains the CMV promoter; the multiple cloning sites and the poly (A) signal sequence. The functionality of pAd-YC and pAd-YC2 was tested by cloning and expression of a chimeric Fc^receptor cDNA and the /3-galactosidase gene. Fcyreceptor expression was confirmed by binding of opsonized erythrocytes to COS-1 infectants; and 각-galactosidase expression was assayed for X-gal staining. The results demonstrate the functional efficacy of the two shuttle vectors. We have since successfully constructed several recombinant adenoviruses by employing the two versatile shuttle vectors. |
| Key Words:
Fcyreceptor,
Recombinant adenovirus,
Shuttle vector |
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