Superscale Plasmid DNA 
Preparation Using Wizard Plus Maxipreps Kit®

Keimyung Medical Journal 1998;17(2):136-140.

Superscale Plasmid DNA Preparation Using Wizard Plus Maxipreps Kit®

Wizard Plus Maxipreps Kit를 이용한 Plasmid DNA의 분리법의 응용

이영재; 한인숙

Abstract

Since the development of molecular biological techniques; the plasmid DNA became indispensable in the research laboratories. Conventionally; the plasmid DNA was purified using CsCl and over 40 hours of high speed centrifugation. To overcome these problems; several manufactures have developed kits for purifying plasmid DNA based on affinity chromatography; however; the yield of single preparation of plasmid DNA was not sufficient for experiments such as gene therapy; gene delivery using liposome; or cDNA vaccination. In this technical report; by precipitating DNA using ethanol and re-using column; we have developed a simple method for preparing several miligrams of plasmid DNA from 300 ml of over night bacterial culture using Wizard Plus Maxipreps Kit ;