| Productions of
Recombinant Tyrosine Kinase B (Trk B) Protein
and its Specific Antibody |
| Tyrosine kinase B (Trk B) 재조합 단백질과 특이 항체 생산 |
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한인숙; 이영재 |
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| Abstract |
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Tyrosine kinase B (Trk B) is the
intracellular receptor for the brain-derived neurotrophic factor (BDNF). In order to study the relationship between the neur?trophin BDNF and its receptor Trk B; the productions of recombinant Trk B proteins were estabilished and the following results were obtained. A partial Trk B gene of rat brain was cloned into E. coli expression vector under the control of T7 promoter. When these partial cDNAs were expressed as recombinant partial Trk B proteins by addition of IPTG; they were accounted for about 20% of total E. coli proteins; displaying a molecular size of 38KDa. Partial Trk B were purified and refolded by metal affinity chromatography and dialysis showing 38KDa as monomer and 76KDa as dimer; respectively. After three times immunizations of rabbit through six weeks; the polyclonal antisera were separated from the blood; and their specificities and titers were investigated by ELISA and immun?histology; respectively.
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| Key Words:
Tyrosine kinase B
(Trk B), recombinant protein, polyclonal antibody.
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