Gene Mutations of Sporadic Advanced Colorectal Cancer in Korean Patients |
한국인 산발적 진행성 대장암의 유전자변이 |
라중호; 박성대; 서민호; 최병길; 서성일; 배옥석; 전소영; 김인숙; 박종욱 |
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Abstract |
To elucidate the molecular
basis of advanced colorectal cancer in Korean patients; genetic alterations of K-ras; p53; and nm23Hl genes in primary colorectal cancer tissues were studied. Eighty tumors and their adjacent mucosa were examined for mutations on K~ms codon 12 by the method of RFLP analysis; using specifically designed primers. /C-ras codon 12 mutations were observed in 25%(20/80) of tumors examined. We have examined 30 cases of human colorectal cancers for the presence of p53 gene mutations in exon 5;7;and 8 of the p53 gene by single strand conformation polymorphism(SSCP) and restriction fragment length polymorphism(RFLP) analysis. Surprisingly; mutation rate of p53 gene was low in mutational hot spots. In addition; there were silent mutations in exon 8(codon 273).
To gain some information on the biologic progression of metastatic colorectal cancer; we have investigated nm23Hl gene by slot blot; PCR-SSCP and PCR-RFLP methods. The 15% (3 of 20) of them exhibited suspicious mutation of nm23Hl gene; whereas in 3 of the 10 colon neoplasms; the nm23 expression was further increased in cancer tissues when compared with their adjacent mucosa. This study suggests different rate of gene mutation between Korea and western countries; therefore p53 hot spots(codon 175; 248; 273) may not be useful for genetic diagnosis and gene therapy purposes. Further studies are needed to obtain information regarding the relationship between advanced colorectal tumors and their genetic behavior.
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Key Words:
Colon cancer, 표-ras,
p53, nm25Hl |
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