| Changes of Femur
Bone Mineral Density and Levels of TNF- a in the Culture Media of Peripheral Blood and Bone Marrow Monocytes after Ovariectomy in Rats |
| 흰쥐에서 난소절제술 시행후 대퇴 골밀도와 말초혈액 및 골수 TNF-α 의 변화에 관한 연구 |
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김성한 |
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| Abstract |
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Although the mechanism of
the increase in bone resorption induced by estrogen deficiency is still controversial; recent studies have suggested that estrogen may modulate the secretion of bone resorption cytokines that are produced in the bone microenvironment and influence bone remodeling. Among them; TNF- o promotes bone resorption by stimulating the activity of mature osteoclasts and osteoclastogenesis; and the production of this cytokine is mediated by estrogen. We performed this study to evaluate the effect of ovariectomy on femur bone mineral density and levels of TNF- a in the culture media of bone marrow monocytes.
The experimental animals were 15 female Sprague-Dswley rats that were 6 weeks of age and weighed an average of 172.8 gm at the beginning of the study. Bilateral ovariectomy were performed in all rats from a vental approach. Bone mineral density(BMD) of femur and levels of TNF- a of oilture media of peripheral blood and bone marrow monocytes were measured before and 8 weeks after operation with using DPX-L and ELISA assay; respectively. Serum levels of LH; FSH; E2 were measured by RIA assay.
BMD of femur was lower after ?variectomy(?.20±0.03 g/cm2) than before ovariectomy(0. 25±0.06 g/cm2)(p<0.05). Serum levels of LH; FSH were higher after ovariectomy(0.71 土0.29 m]U/ml; 10.27±L21 mIU/ml) than before ovariectomy(0.17±0.04 mIU/ml;2.79±0.61 mIU/ml) (p<0.01) and serum level of E2 was lower after ovariect?my(6.37±4.75 pg/ml) than before ovariectomy(7159.16 ± 385.82 pg/ml)(p<0;01);respectively. Levels of TNF- a in culture media of bone marrow monocytes were higher after ovariectomy(59.26±5.65 pg/ml) than before 'ovariectomy(23.37±4.13 pg/ml)(p<0.01); but there were no statistically significant differences in culture media of the peripheral blood monocytes(before ovariectomy： 22.96±3.62 pg/ml ; after ovariectomy： 23.55土 2;98pg/ml)( p〉0.05).
It is possible that increased production of TNF-o in estrogen deficiency induced by ovariectomy occures in the bone microenvironment and this cytokine may play a critical causal role in inducing bone loss.
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| Key Words:
Ovariectomy,
TNF- a, BMD
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