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Keimyung Medical Journal 2014;33(2):109-119.
Published online December 31, 2014.
Sonication Induces Apoptosis in HL-60 Cells.
Moonyoung Choi, Incheol Seo, Seong Il Suh, Min Ho Suh, Won Ki Baek
Department of Microbiology, Keimyung University School of Medicine, Daegu, Korea. wonki@dsmc.or.kr
Abstract
To investigate a specific mechanism of apoptosis induced by sonication, we applied 20 kHz ultrasound to leukemia cell line HL-60 with different intensities (0-60 W/cm2) and time durations (0-100 sec). In accordance with previous reports, ultrasound treatment in HL-60 cells induced immediate cell death and delayed cell death which are associated with cell lysis and apoptosis, respectively. Delayed cell death of HL-60 was also detected 5 hours after sonication in our experiment. Detection of caspase activation by Western blot and sub-G1 accumulation by flow cytometry confirmed that apoptosis plays a role in delayed cell death induced by sonication in HL-60 cells. In addition, we found that decrease in lysosomes of HL-60 cells after sonication suggesting lysosomal rupture is involved in the mechanism of cell death induced by sonication.
Key Words: Apoptosis, Leukemia, Lysosome, Sonication, Ultrasonic therapy
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