An Experimental Study of Human Amniotic Membrane as a Topical Hemostatic in Canine Liver and Splenic Injury

Keimyung Medical Journal 1988;7(2):330-334.

간장 및 비장손상에서 국소지혈제로써의 인체양막의 이용에 대한 실험적 연구

손수상; 김인호; 강중신

Abstract

The hemostatic mechanism of action of collagen appears to involve both activation of the intrinsic clotting mechanism via factors XII and XI and a platelet interaction consisting of adherence and aggregation of platelets via adenosine diphosphate release. Of many commercially available local hemostats; the microcrystalline collagenCAvitene) is known to be one of the most effective agents. Human amniotic membrane contains collagen that initiates platelet aggregation and activation of the intrinsic pathway of the clotting mechanism. The authors studied the hemostatic effectiveness of the human amniotic membrane and the results were compared with that of Avitene web; Collastat and Gelfoam. Canine liver and splenic bleeding models were used. Human amniotic membrane and Avitene web had similar effectiveness(97.2%) and were more effective than Collastat(77.8%) or Gelfoam(6X.l%). First application success rates also revealed that amniotic membrane(80.6%) and Avitene web(77.8%) were more successful than others(47%). Humam amniotic membrane is an effective topical hemostatic agent in experimental canine liver and splenic wounds when compared with several currently used agents and can also result in cost savings.