| Effect of Common Bile
Duct Ligation on Hepatic Aryl Sulfotransferase 1;11 Isozyme Activity in Chronic Ethanol Intoxicated Rats
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| 만성 주정 중독 쥐에서 총담관 결찰이 간의 Aryl Sulfotransferase I;II Isozyme의 활성에 미치는 영향 |
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조경일; 곽춘식 |
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| Abstract |
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The drinking hazard in
hepatobiliary disease was studied by the assays of liver cytosolic; mitochondrial; and microsomal aryl sulfotransferase I; II isozyme; in cholestasis induced by common bile duct (CBD) ligation after chronic ethanol intoxication with rats. The activity of serum aryl sulfotrans-ferase I;II isozyme was also measured.
Liver mitochondrial aryl sulfotransferase I;II isozyme activity in group of CBD ligation after chronic ethanoJ intoxication showed a greater decrease between the 3rd and the 14th day after CBD ligation than that in group of CBD ligation alone. However; the cytosolic I;II isozyme activity did not change?
The microsomal aryl sulfotransferase I ;11 isozyme activity in the CBD ligation after chronic ethanol intoxication showed a greater decrease between the 7th and the 14th day after CBD ligation than that in the CBD ligation alone.
The serum aryl sulfotrnasferase I;II isozyme activity in the CBD ligation after chronic ethanol intoxication showed a greater increase between the 2nd and the 14th day after CBD ligation than that in the CBD ligation alone.
The Vmax values of the liver mitochondrial and microsomal aryl sulfotransferase I;II isozymes in the CBD ligation after chronic ethanol intoxication showed a greater decrease at the 14th day after CBD ligation than that in the CBD ligation alone. However; the Km values of the above hepatic enzymes did not change.
The results indicate that the biosynthesis of the hepatic aryl sulfotransferase I; II isozyme is decreasing in chronic ethanol intoxication with cholestasis than in cholestasis alone. The increased activity of the serum aryl sulfotransferase I f II isozyme in chronic ethanol intoxi-
cation with cholestasis than in cholestasis alone reflect that increased hepatic damages which cause this isozyme to leak into the blood in great quantity.
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| Key Words:
Alcoholic intoxication,
Aryl sulfotransferase,
Cholestasis, extrahepatic |
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